Product at a glance

  • Liquid product designed to help combat odours associated with reduced sulphur compounds.
  • Mixture of chemical and biological component works synergistically to impact sulphate reducing bacteria.
  • Chemical component gives bacteria a better option for metabolic activities which leads to nitrogen generation instead of sulphide.
  • Biological component outcompetes sulphate reducing bacteria for resources and space.
  • Can impact the formation of biofilms upon surfaces that may harbour sulphate reducing bacteria.
  • Broad metabolic capability of Bacillus consortia able to degrade wide range of COD sources including Fats, Oils and Grease.
  • Can help improve effluent parameters as well as tackling odours.
  • Non-pathogenic, environmentally responsible and easy to use.
  • Robust heterotrophic growth parameters allow for growth across a range of conditions conferring resilience against system shocks.
  • Manufactured in accordance with recognised international standard ISO 9001 to guarantee quality, integrity and reproducibility.
  • Can be used to improve plant performance to meet emissions directives.
+44 (0)29 2079 1185 info@genesisbiosciences.com genesisbiosciences.co.uk

Unit P1, Capital Business Park,
Capital Point, Parkway,
Cardiff CF3 2PU UK

Applications

Municipal and industrial wastewater plants

Lagoons and ponds

Cess and slurry pits

Tackling sulphate reducing biofiling

Areas suffering with anaerobic issues

The technology

Evogen Sulphide Controller has been specially formulated to tackle odours and issues associated with reduced sulphur compounds (rotten egg smell) within oxygen depleted zones.

The product is a liquid that features a sodium nitrate base combined with different Bacillus species with a diverse metabolic capability.

How it works

Evogen Sulphide Controller uses both a chemical component and a biological component to deliver a multi-pronged challenge to the most common cause of reduced sulphur compounds – sulfate reducing bacteria (SRB).

The delivery of sodium nitrate into zones favouring sulphate reduction offers the resident microbes a better, higher energy electron acceptor. This means the bacteria can gain more from their food using nitrate rather than sulphate. This decreases sulphate reduction because instead of ‘breathing’ sulphate the microbes instead breathe nitrate, and thus, instead of expelling reduced sulphur compounds the bacteria produce nitrogen via denitrification.

The biological component contains a blend of Bacillus strains that have been selected through both laboratory and genomic methods to outcompete and disrupt the growth of sulphate reducing bacteria (SRB). The diverse metabolic capability of the Bacillus combined with a wide range of extracellular enzymes ensure that potential fuel for SRB is strongly competed for. Furthermore, a broad range of antimicrobial secondary metabolites expressed by the Bacillus help suppress the growth of SRB. In turn, this could prevent corrosion, particularly with regards to biofilms formed upon steel and metal surfaces, where dosing of the Bacillus consortia within Evogen Sulphide Controller has been shown to lower biofilm coverage and interfere with biofilm-based sulphate reduction.

In addition to helping to combat reduced sulphur odours, the product will also help with improving effluent parameters by contributing to BOD/COD removal as well as impacting a range of other potential odour compounds such as volatile fatty acids like propionic and valeric acid.

Directions for use

Evogen Sulphide Controller is supplied in 20L, 200L and 1,000L containers and should be dosed directly into the effluent downstream from the problem area.

Dose at a treatment ratio of between 0.6 to 2.0 times the concentration of sulphide in the system. Treatment will vary with conditions, start with a higher end and then work down. Ensure a minimum initial retention time of 3 hours to get the bacteria to switch over to nitrate based respiration. Continue to dose for up 2-4 weeks after the odour has been eliminated to ensure the sulphate reducing bacteria are adequately outcompeted.

As the product contains nitrate, care should be taken to ensure enough retention time and appropriate conditions are present within the system to account for the extra nitrogen. Our technical support team are able to provide assistance where necessary.

Considerations

Ideally suited towards effluent rich in organic materials such as proteins, fats, oils and grease, biomass and carbohydrates. Is able to degrade a range of volatile fatty acids and impact biofilms such as those formed upon clarifier plates. Can be used in areas of odour including wet wells and septic tanks, where liquid retention times permit.

Where it is inappropriate to dose a nitrate containing product, we suggest trying our Evogen Clarity which is a blend of Bacillus combined with a chemical carrier that slowly releases oxygen into a system, helping to aerate deep anoxic areas shutting down sulphate reduction and the generation of odorous compounds.

Limitations

The product will perform poorly in highly alkaline (>pH 9) and highly acidic (<pH 4) effluents and will also lose activity at low (<15 ̊C) and high (>40 ̊C) temperatures. Effluent types should have appropriate carbon and phosphorous content to support microbial growth.

For specialised effluent types contact our technical department for more information.

Product specification

Count
1E+9 CFU/g Bacillus

Appearance
Tan coloured liquid

Fragrance
Fresh (eucalyptus and mint)

pH
pH 7.0 – 8.8

Shelf life
24 month (unopened). If opened store in a cool dry place to maintain product integrity

Environmental conditions

Salinity
Freshwater to seawater and above (no impact upon performance between 0 - 50 g/L)

pH
pH 5 – to pH 8.5 (Optimum between pH 6 and pH 7.5)

Temperature
10 ̊C – 40 ̊C (Optimum between 20 ̊C and 35 ̊C)

Effluent type
Organic rich effluents, (municipal, paper and pulp, dairy, bakery)

Safety

Via a combination of cell cytotoxicity assays and genomic screens for toxin production, we can confirm that all Bacillus species and strains within the product are safe to use, should harbour no toxicity towards humans or animals and do not have a negative impact upon the environment.

From analysing the DNA of our Bacillus strains we can confirm a lack of mobile genetic elements, which strongly indicates a low potential for transmission of antimicrobial resistance (AMR) genes.

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